Cotemporal Single-Molecule Force and Fluorescence Measurements to Determine the Mechanism of Ribosome Translocation.

Desai, Varsha P; Frank, Filipp; Bustamante, Carlos J

Desai, Varsha P; Frank, Filipp; Bustamante, Carlos J.

Affiliation

Desai VP; University of California, Los Angeles, Los Angeles, CA, USA.
Frank F; Emory University, Atlanta, GA, USA. Filipp.Frank@emory.edu.
Bustamante CJ; University of California and Howard Hughes Medical Institute, Berkeley, Berkeley, CA, USA. CarlosB@berkeley.edu.

Methods Mol Biol ; 2478: 381-399, 2022.

Article in En | MEDLINE | ID: mdl-36063328

Subject(s)

Escherichia coli; Peptide Elongation Factor G; Escherichia coli/genetics; Peptide Elongation Factor G/analysis; Peptide Elongation Factor G/genetics; Peptide Elongation Factor G/metabolism; Protein Biosynthesis; RNA, Messenger/metabolism; Ribosomes/metabolism

Key words

Fluorescence; Optical tweezers; Ribosome; Single-molecule; Translation; mRNA Unwinding

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Peptide Elongation Factor G / Escherichia coli Language: En Journal: Methods Mol Biol Journal subject: BIOLOGIA MOLECULAR Year: 2022 Type: Article Affiliation country: United States

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