Automated assays for trace elements and ferritin measurement in saliva of pigs: Analytical validation and a pilot application to evaluate different iron status.
Res Vet Sci
; 152: 410-416, 2022 Dec 20.
Article
in En
| MEDLINE
| ID: mdl-36116417
ABSTRACT
The aim of this study was to validate automated methods to measure iron (Fe), zinc (Zn), copper (Cu) and ferritin in pig saliva samples. A complete analytical validation was performed of all assays. In addition, these methods were applied to saliva of Fe supplemented (n = 22) and non-supplemented (n = 20) piglets. All assays were able to measure these biomarkers in pig saliva with adequate precision, accuracy and high sensitivity and, in case of trace elements without needing a deproteinization pre-process. The group of piglets supplemented with Fe presented significantly higher levels of ferritin and Zn in saliva. In conclusion, the automated assays evaluated were able to measure Fe, Zn, Cu and ferritin in saliva of pigs, and in case of trace elements, they have the advantage of not needing a deproteinization pre-treatment and thus these analytes can be measured in a simple and fast manner.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Trace Elements
Limits:
Animals
Language:
En
Journal:
Res Vet Sci
Year:
2022
Type:
Article
Affiliation country:
Spain