Effect of CD34⺠Total/Myeloid CD34⺠Cell Progenitors and B-Lymphoid Progenitors Within the Bone Marrow Grafts on the Hematopoietic Recovery After Hematopoietic Stem Cell Transplantation.
Exp Clin Transplant
; 20(10): 937-944, 2022 10.
Article
in En
| MEDLINE
| ID: mdl-36409053
ABSTRACT
OBJECTIVES:
The influence of CD34⺠cells on transplant outcomes following hematopoietic stem cell transplantation remains controversial. A minimum of 2.0 to 2.5 million CD34⺠cells/kg of patient weight is requested for a rapid and durable engraftment. The aim of this study was to detect the ratio of CD34+ B-lymphoid progenitors (hematogones) in bone marrow grafts and investigate their effects on hematopoietic recovery after transplant. MATERIALS ANDMETHODS:
Our study included 41 patients who received a bone marrow graft from their HLA-matched donor from 2016 through 2019. The CD34⺠cell numbers within the graft were detected using Stem-Kit (Beckman Coulter). The ratio of CD34⺠hematogones was determined either by their light scatter characteristics or by the detection of CD34, CD19, or CD10 coexpressing cells in a separate tube.RESULTS:
The median number of CD34⺠cells was 5.9 × 106/kg (0.8-14.3 × 106/kg). The CD34⺠cells consisted of 71% (range, 35.7%-100%) and 29% (range, 5.7%-64.3%) myeloid and B-lymphoid progenitors, respectively. Percentage of CD34⺠(P < .001) and total (P < .001) hematogones in correlation with donor age. Time of neutrophil engraftment was significantly longer (P = .039) when total infused CD34⺠cell content was <3 × 106/kg.CONCLUSIONS:
A remarkable population of hematogones within the CD34⺠cell pool was detected in bone marrow grafts. Detection of the ratio of hematogones and most primitive stem cells (CD34âºCD90âºCD38â») may overall provide more information to build a better correlation between CD34⺠cell content and the recovery of bone marrow.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Bone Marrow
/
Hematopoietic Stem Cell Transplantation
Limits:
Humans
Language:
En
Journal:
Exp Clin Transplant
Journal subject:
TRANSPLANTE
Year:
2022
Type:
Article
Affiliation country:
Turkey