Sifting through the core-genome to identify putative cross-protective antigens against Riemerella anatipestifer.
Appl Microbiol Biotechnol
; 107(9): 3085-3098, 2023 May.
Article
in En
| MEDLINE
| ID: mdl-36941438
ABSTRACT
Infectious serositis of ducks, caused by Riemerella anatipestifer, is one of the main infectious diseases that harm commercial ducks. Whole-strain-based vaccines with no or few cross-protection were observed between different serotypes of R. anatipestifer, and so far, control of infection is hampered by a lack of effective vaccines, especially subunit vaccines with cross-protection. Since the concept of reverse vaccinology was introduced, it has been widely used to screen for protective antigens in important pathogens. In this study, pan-genome binding reverse vaccinology, an emerging approach to vaccine candidate screening, was used to screen for cross-protective antigens against R. anatipestifer. Thirty proteins were identified from the core-genome as potential cross-protective antigens. Three of these proteins were recombinantly expressed, and their immunoreactivity with five antisera (anti-serotypes 1, 2, 6, 10, and 11) was demonstrated by Western blotting. Our study established a method for high-throughput screening of cross-protective antigens against R. anatipestifer in silico, which will lay the foundation for the development of a cross-protective subunit vaccine controlling R. anatipestifer infection. KEY POINTS ⢠Pan-genome binding reverse vaccine approach was first established in R. anatipestifer to screen for subunit vaccine candidates. ⢠Thirty potential cross-protective antigens against R. anatipestifer were identified by this method. ⢠The reliability of the method was verified preliminarily by the results of Western blotting of three of these potential antigens.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Poultry Diseases
/
Flavobacteriaceae Infections
/
Riemerella
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
Appl Microbiol Biotechnol
Year:
2023
Type:
Article
Affiliation country:
China