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Fibroblastic reticular cells provide a supportive niche for lymph node-resident macrophages.
D'Rozario, Joshua; Knoblich, Konstantin; Lütge, Mechthild; Shibayama, Christian Pérez; Cheng, Hung-Wei; Alexandre, Yannick O; Roberts, David; Campos, Joana; Dutton, Emma E; Suliman, Muath; Denton, Alice E; Turley, Shannon J; Boyd, Richard L; Mueller, Scott N; Ludewig, Burkhard; Heng, Tracy S P; Fletcher, Anne L.
Affiliation
  • D'Rozario J; Department of Biochemistry and Molecular Biology, and Monash Biomedicine Discovery Institute, Monash University, Clayton, Australia.
  • Knoblich K; Department of Anatomy and Developmental Biology, Monash Biomedicine Discovery Institute, Monash University, Clayton, Australia.
  • Lütge M; Institute of Immunology and Immunotherapy, University of Birmingham, Birmingham, UK.
  • Shibayama CP; Department of Biochemistry and Molecular Biology, and Monash Biomedicine Discovery Institute, Monash University, Clayton, Australia.
  • Cheng HW; Institute of Immunology and Immunotherapy, University of Birmingham, Birmingham, UK.
  • Alexandre YO; Institute of Immunobiology, Kantonsspital St. Gallen, St. Gallen, Switzerland.
  • Roberts D; Institute of Immunobiology, Kantonsspital St. Gallen, St. Gallen, Switzerland.
  • Campos J; Institute of Immunobiology, Kantonsspital St. Gallen, St. Gallen, Switzerland.
  • Dutton EE; Department of Microbiology and Immunology, The Peter Doherty Institute for Infection and Immunity, The University of Melbourne, VIC, Melbourne, Australia.
  • Suliman M; Institute of Immunology and Immunotherapy, University of Birmingham, Birmingham, UK.
  • Denton AE; Institute of Inflammation and Ageing, University of Birmingham, Birmingham, UK.
  • Turley SJ; Institute of Immunology and Immunotherapy, University of Birmingham, Birmingham, UK.
  • Boyd RL; Institute of Immunology and Immunotherapy, University of Birmingham, Birmingham, UK.
  • Mueller SN; Department of Immunology and Inflammation, Imperial College London, London, UK.
  • Ludewig B; Department of Cancer Immunology, Genentech Inc., South San Francisco, CA, USA.
  • Heng TSP; Cartherics Pty Ltd, Hudson Institute for Medical Research, Clayton, Australia.
  • Fletcher AL; Department of Microbiology and Immunology, The Peter Doherty Institute for Infection and Immunity, The University of Melbourne, VIC, Melbourne, Australia.
Eur J Immunol ; 53(9): e2250355, 2023 09.
Article in En | MEDLINE | ID: mdl-36991561
The lymph node (LN) is home to resident macrophage populations that are essential for immune function and homeostasis, but key factors controlling this niche are undefined. Here, we show that fibroblastic reticular cells (FRCs) are an essential component of the LN macrophage niche. Genetic ablation of FRCs caused rapid loss of macrophages and monocytes from LNs across two in vivo models. Macrophages co-localized with FRCs in human LNs, and murine single-cell RNA-sequencing revealed that FRC subsets broadly expressed master macrophage regulator CSF1. Functional assays containing purified FRCs and monocytes showed that CSF1R signaling was sufficient to support macrophage development. These effects were conserved between mouse and human systems. These data indicate an important role for FRCs in maintaining the LN parenchymal macrophage niche.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Signal Transduction / Fibroblasts Type of study: Prognostic_studies Limits: Animals / Humans Language: En Journal: Eur J Immunol Year: 2023 Type: Article Affiliation country: Australia
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Signal Transduction / Fibroblasts Type of study: Prognostic_studies Limits: Animals / Humans Language: En Journal: Eur J Immunol Year: 2023 Type: Article Affiliation country: Australia