Protocol for tumor dissociation and fluorescence-activated cell sorting of human head and neck cancers.
STAR Protoc
; 4(2): 102294, 2023 May 06.
Article
in En
| MEDLINE
| ID: mdl-37149858
ABSTRACT
Tumors originating from the head and neck represent diverse histologies and are comprised of several cell types, including malignant cells, cancer-associated fibroblasts, endothelial cells, and immune cells. In this protocol, we describe a step-by-step approach for the dissociation of fresh human head and neck tumor specimens, followed by isolation of viable single cells using fluorescence-activated cell sorting. Our protocol facilitates the effective downstream use of techniques, including single-cell RNA sequencing and generation of three-dimensional patient-derived organoids. For complete details on the use and execution of this protocol, please refer to Puram et al. (2017)1 and Parikh et al. (2022).2.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Language:
En
Journal:
STAR Protoc
Year:
2023
Type:
Article
Affiliation country:
United States