Bovine milk-derived exosomes attenuate NLRP3 inflammasome and NF-κB signaling in the lung during neonatal necrotizing enterocolitis.
Pediatr Surg Int
; 39(1): 211, 2023 Jun 03.
Article
in En
| MEDLINE
| ID: mdl-37268798
ABSTRACT
PURPOSE:
Necrotizing enterocolitis (NEC), an inflammatory intestinal disease common in premature infants, has been associated with the development of lung damage. Toll-like receptor 4 has been shown to regulate inflammation in the NEC lungs, however, other important inflammatory mechanisms have not been thoroughly investigated. In addition, we reported that milk-derived exosomes were able to attenuate intestinal injury and inflammation in experimental NEC. This study aims to (i) investigate the role of the NLRP3 inflammasome and NF-κB pathway in regulating lung damage during experimental NEC; and (ii) evaluate the therapeutic potential of bovine milk exosomes in reducing lung inflammation and injury during NEC.METHODS:
NEC was induced by gavage feeding of hyperosmolar formula, hypoxia, and lipopolysaccharide administration in neonatal mice from postnatal days 5-9. Exosomes were obtained by ultracentrifugation of bovine milk and administered during each formula feed.RESULTS:
The lung of NEC pups showed increased inflammation, tissue damage, NLRP3 inflammasome expression, and NF-κB pathway activation, which were attenuated upon exosome administration.CONCLUSION:
Our findings suggest that the lung undergoes significant inflammation and injury following experimental NEC which are attenuated by bovine milk-derived exosomes. This emphasizes the therapeutic potential of exosomes not just on the intestine but also on the lung.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Enterocolitis, Necrotizing
/
Exosomes
/
Infant, Newborn, Diseases
Type of study:
Prognostic_studies
Limits:
Animals
/
Humans
/
Newborn
Language:
En
Journal:
Pediatr Surg Int
Journal subject:
PEDIATRIA
Year:
2023
Type:
Article
Affiliation country:
Canada