Single-cell spatial transcriptome reveals cell-type organization in the macaque cortex.
Cell
; 186(17): 3726-3743.e24, 2023 08 17.
Article
in En
| MEDLINE
| ID: mdl-37442136
ABSTRACT
Elucidating the cellular organization of the cerebral cortex is critical for understanding brain structure and function. Using large-scale single-nucleus RNA sequencing and spatial transcriptomic analysis of 143 macaque cortical regions, we obtained a comprehensive atlas of 264 transcriptome-defined cortical cell types and mapped their spatial distribution across the entire cortex. We characterized the cortical layer and region preferences of glutamatergic, GABAergic, and non-neuronal cell types, as well as regional differences in cell-type composition and neighborhood complexity. Notably, we discovered a relationship between the regional distribution of various cell types and the region's hierarchical level in the visual and somatosensory systems. Cross-species comparison of transcriptomic data from human, macaque, and mouse cortices further revealed primate-specific cell types that are enriched in layer 4, with their marker genes expressed in a region-dependent manner. Our data provide a cellular and molecular basis for understanding the evolution, development, aging, and pathogenesis of the primate brain.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Cerebral Cortex
/
Single-Cell Analysis
/
Transcriptome
/
Macaca
Limits:
Animals
/
Humans
Language:
En
Journal:
Cell
Year:
2023
Type:
Article
Affiliation country:
China