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Multiphoton intravital microscopy of rodents.
Scheele, Colinda L G J; Herrmann, David; Yamashita, Erika; Celso, Cristina Lo; Jenne, Craig N; Oktay, Maja H; Entenberg, David; Friedl, Peter; Weigert, Roberto; Meijboom, Franck L B; Ishii, Masaru; Timpson, Paul; van Rheenen, Jacco.
Affiliation
  • Scheele CLGJ; Laboratory for Intravital Imaging and Dynamics of Tumor Progression, VIB Center for Cancer Biology, KU Leuven, Leuven, Belgium.
  • Herrmann D; Department of Oncology, KU Leuven, Leuven, Belgium.
  • Yamashita E; Cancer Ecosystems Program, Garvan Institute of Medical Research and The Kinghorn Cancer Centre, Cancer Department, Sydney, New South Wales, Australia.
  • Celso CL; St. Vincent's Clinical School, Faculty of Medicine, UNSW Sydney, Sydney, New South Wales, Australia.
  • Jenne CN; Department of Immunology and Cell Biology, Graduate School of Medicine and Frontier Biosciences, Osaka University, Osaka, Japan.
  • Oktay MH; WPI-Immunology Frontier Research Center, Osaka University, Osaka, Japan.
  • Entenberg D; Laboratory of Bioimaging and Drug Discovery, National Institutes of Biomedical Innovation, Health and Nutrition, Osaka, Japan.
  • Friedl P; Department of Life Sciences and Centre for Hematology, Imperial College London, London, UK.
  • Weigert R; Sir Francis Crick Institute, London, UK.
  • Meijboom FLB; Snyder Institute for Chronic Diseases, University of Calgary, Calgary, Alberta, Canada.
  • Ishii M; Department of Pathology, Albert Einstein College of Medicine/Montefiore Medical Center, Bronx, NY, USA.
  • Timpson P; Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine/Montefiore Medical Center, Bronx, NY, USA.
  • van Rheenen J; Integrated Imaging Program, Albert Einstein College of Medicine/Montefiore Medical Center, Bronx, NY, USA.
Nat Rev Methods Primers ; 22022.
Article in En | MEDLINE | ID: mdl-37621948
ABSTRACT
Tissues are heterogeneous with respect to cellular and non-cellular components and in the dynamic interactions between these elements. To study the behaviour and fate of individual cells in these complex tissues, intravital microscopy (IVM) techniques such as multiphoton microscopy have been developed to visualize intact and live tissues at cellular and subcellular resolution. IVM experiments have revealed unique insights into the dynamic interplay between different cell types and their local environment, and how this drives morphogenesis and homeostasis of tissues, inflammation and immune responses, and the development of various diseases. This Primer introduces researchers to IVM technologies, with a focus on multiphoton microscopy of rodents, and discusses challenges, solutions and practical tips on how to perform IVM. To illustrate the unique potential of IVM, several examples of results are highlighted. Finally, we discuss data reproducibility and how to handle big imaging data sets.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Language: En Journal: Nat Rev Methods Primers Year: 2022 Type: Article Affiliation country: Belgium
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Full text: 1 Collection: 01-internacional Database: MEDLINE Language: En Journal: Nat Rev Methods Primers Year: 2022 Type: Article Affiliation country: Belgium