Single-cell transcriptome analysis of the germ cells and somatic cells during mitotic quiescence stage in goats.
FASEB J
; 37(11): e23244, 2023 11.
Article
in En
| MEDLINE
| ID: mdl-37823602
ABSTRACT
The mitotic quiescence of prospermatogonia is the event known to occur during genesis of the male germline and is tied to the development of the spermatogenic lineage. The regulatory mechanisms and the functional importance of this process have been demonstrated in mice; however, regulation of this process in human and domestic animal is still largely unknown. In this study, we employed single-cell RNA sequencing to identify transcriptional signatures of prospermatogonia and major somatic cell types in testes of goats at E85, E105, and E125. We identified both common and specific Gene Ontology categories, transcription factor regulatory networks, and cell-cell interactions in cell types from goat testis. We also analyzed the transcriptional dynamic changes in prospermatogonia, Sertoli cells, Leydig cells, and interstitial cells. Our datasets provide a useful resource for the study of domestic animal germline development.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Goats
/
Single-Cell Gene Expression Analysis
Type of study:
Prognostic_studies
Limits:
Animals
/
Humans
/
Male
Language:
En
Journal:
FASEB J
Journal subject:
BIOLOGIA
/
FISIOLOGIA
Year:
2023
Type:
Article
Affiliation country:
China