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LSM14B is essential for oocyte meiotic maturation by regulating maternal mRNA storage and clearance.
Wan, Yanling; Yang, Shuang; Li, Tongtong; Cai, Yuling; Wu, Xinyue; Zhang, Mingyu; Muhammad, Tahir; Huang, Tao; Lv, Yue; Chan, Wai-Yee; Lu, Gang; Li, Jingxin; Sha, Qian-Qian; Chen, Zi-Jiang; Liu, Hongbin.
Affiliation
  • Wan Y; Center for Reproductive Medicine, Shandong University, Jinan, Shandong 250012, China.
  • Yang S; Key Laboratory of Reproductive Endocrinology of Ministry of Education, Shandong University, Jinan, Shandong 250012, China.
  • Li T; Shandong Provincial Clinical Medicine Research Center for Reproductive Health, Jinan, Shandong 250012, China.
  • Cai Y; State Key Laboratory of Reproductive Medicine and Offspring Health, Jinan, Shandong 250012, China.
  • Wu X; Department of Physiology School of Basic Medical Sciences, Cheeloo College of Medicine, Shandong University, Jinan, Shandong 250012, China.
  • Zhang M; Center for Reproductive Medicine, Shandong University, Jinan, Shandong 250012, China.
  • Muhammad T; Key Laboratory of Reproductive Endocrinology of Ministry of Education, Shandong University, Jinan, Shandong 250012, China.
  • Huang T; Shandong Provincial Clinical Medicine Research Center for Reproductive Health, Jinan, Shandong 250012, China.
  • Lv Y; State Key Laboratory of Reproductive Medicine and Offspring Health, Jinan, Shandong 250012, China.
  • Chan WY; Center for Reproductive Medicine, Shandong University, Jinan, Shandong 250012, China.
  • Lu G; Key Laboratory of Reproductive Endocrinology of Ministry of Education, Shandong University, Jinan, Shandong 250012, China.
  • Li J; Shandong Provincial Clinical Medicine Research Center for Reproductive Health, Jinan, Shandong 250012, China.
  • Sha QQ; State Key Laboratory of Reproductive Medicine and Offspring Health, Jinan, Shandong 250012, China.
  • Chen ZJ; Center for Reproductive Medicine, Shandong University, Jinan, Shandong 250012, China.
  • Liu H; Key Laboratory of Reproductive Endocrinology of Ministry of Education, Shandong University, Jinan, Shandong 250012, China.
Nucleic Acids Res ; 51(21): 11652-11667, 2023 Nov 27.
Article in En | MEDLINE | ID: mdl-37889087
ABSTRACT
Fully grown oocytes remain transcriptionally quiescent, yet many maternal mRNAs are synthesized and retained in growing oocytes. We now know that maternal mRNAs are stored in a structure called the mitochondria-associated ribonucleoprotein domain (MARDO). However, the components and functions of MARDO remain elusive. Here, we found that LSM14B knockout prevents the proper storage and timely clearance of mRNAs (including Cyclin B1, Btg4 and other mRNAs that are translationally activated during meiotic maturation), specifically by disrupting MARDO assembly during oocyte growth and meiotic maturation. With decreased levels of storage and clearance, the LSM14B knockout oocytes failed to enter meiosis II, ultimately resulting in female infertility. Our results demonstrate the function of LSM14B in MARDO assembly, and couple the MARDO with mRNA clearance and oocyte meiotic maturation.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Oogenesis / RNA, Messenger, Stored Limits: Animals / Female / Humans / Male Language: En Journal: Nucleic Acids Res Year: 2023 Type: Article Affiliation country: China
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Oogenesis / RNA, Messenger, Stored Limits: Animals / Female / Humans / Male Language: En Journal: Nucleic Acids Res Year: 2023 Type: Article Affiliation country: China