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Atlas of fetal metabolism during mid-to-late gestation and diabetic pregnancy.
Perez-Ramirez, Cesar A; Nakano, Haruko; Law, Richard C; Matulionis, Nedas; Thompson, Jennifer; Pfeiffer, Andrew; Park, Junyoung O; Nakano, Atsushi; Christofk, Heather R.
Affiliation
  • Perez-Ramirez CA; Department of Biological Chemistry, David Geffen School of Medicine, University of California, Los Angeles (UCLA), Los Angeles, CA 90095, USA; Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, UCLA, Los Angeles, CA 90095, USA.
  • Nakano H; Department of Molecular, Cell, and Developmental Biology, UCLA, Los Angeles, CA 90095, USA.
  • Law RC; Department of Chemical and Biomolecular Engineering, UCLA, Los Angeles, CA 90095, USA.
  • Matulionis N; Department of Biological Chemistry, David Geffen School of Medicine, University of California, Los Angeles (UCLA), Los Angeles, CA 90095, USA.
  • Thompson J; Department of Molecular, Cell, and Developmental Biology, UCLA, Los Angeles, CA 90095, USA.
  • Pfeiffer A; Department of Biological Chemistry, David Geffen School of Medicine, University of California, Los Angeles (UCLA), Los Angeles, CA 90095, USA.
  • Park JO; Department of Chemical and Biomolecular Engineering, UCLA, Los Angeles, CA 90095, USA; Jonsson Comprehensive Cancer Center, UCLA, Los Angeles, CA 90095, USA; Molecular Biology Institute, UCLA, Los Angeles, CA 90095, USA.
  • Nakano A; Department of Molecular, Cell, and Developmental Biology, UCLA, Los Angeles, CA 90095, USA; Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, UCLA, Los Angeles, CA 90095, USA; Molecular Biology Institute, UCLA, Los Angeles, CA 90095, USA; Department of Cell Physiology, The
  • Christofk HR; Department of Biological Chemistry, David Geffen School of Medicine, University of California, Los Angeles (UCLA), Los Angeles, CA 90095, USA; Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, UCLA, Los Angeles, CA 90095, USA; Jonsson Comprehensive Cancer Center, UCLA, Los
Cell ; 187(1): 204-215.e14, 2024 01 04.
Article in En | MEDLINE | ID: mdl-38070508
ABSTRACT
Mounting evidence suggests metabolism instructs stem cell fate decisions. However, how fetal metabolism changes during development and how altered maternal metabolism shapes fetal metabolism remain unexplored. We present a descriptive atlas of in vivo fetal murine metabolism during mid-to-late gestation in normal and diabetic pregnancy. Using 13C-glucose and liquid chromatography-mass spectrometry (LC-MS), we profiled the metabolism of fetal brains, hearts, livers, and placentas harvested from pregnant dams between embryonic days (E)10.5 and 18.5. Our analysis revealed metabolic features specific to a hyperglycemic environment and signatures that may denote developmental transitions during euglycemic development. We observed sorbitol accumulation in fetal tissues and altered neurotransmitter levels in fetal brains isolated from hyperglycemic dams. Tracing 13C-glucose revealed disparate fetal nutrient sourcing depending on maternal glycemic states. Regardless of glycemic state, histidine-derived metabolites accumulated in late-stage fetal tissues. Our rich dataset presents a comprehensive overview of in vivo fetal tissue metabolism and alterations due to maternal hyperglycemia.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Diabetes, Gestational / Diabetes Mellitus / Fetus Limits: Animals / Pregnancy Language: En Journal: Cell Year: 2024 Type: Article Affiliation country: United States
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Diabetes, Gestational / Diabetes Mellitus / Fetus Limits: Animals / Pregnancy Language: En Journal: Cell Year: 2024 Type: Article Affiliation country: United States