Your browser doesn't support javascript.
loading
Diverse array of neutralizing antibodies elicited upon Spike Ferritin Nanoparticle vaccination in rhesus macaques.
Sankhala, Rajeshwer S; Lal, Kerri G; Jensen, Jaime L; Dussupt, Vincent; Mendez-Rivera, Letzibeth; Bai, Hongjun; Wieczorek, Lindsay; Mayer, Sandra V; Zemil, Michelle; Wagner, Danielle A; Townsley, Samantha M; Hajduczki, Agnes; Chang, William C; Chen, Wei-Hung; Donofrio, Gina C; Jian, Ningbo; King, Hannah A D; Lorang, Cynthia G; Martinez, Elizabeth J; Rees, Phyllis A; Peterson, Caroline E; Schmidt, Fabian; Hart, Tricia J; Duso, Debra K; Kummer, Lawrence W; Casey, Sean P; Williams, Jazmean K; Kannan, Shruthi; Slike, Bonnie M; Smith, Lauren; Swafford, Isabella; Thomas, Paul V; Tran, Ursula; Currier, Jeffrey R; Bolton, Diane L; Davidson, Edgar; Doranz, Benjamin J; Hatziioannou, Theodora; Bieniasz, Paul D; Paquin-Proulx, Dominic; Reiley, William W; Rolland, Morgane; Sullivan, Nancy J; Vasan, Sandhya; Collins, Natalie D; Modjarrad, Kayvon; Gromowski, Gregory D; Polonis, Victoria R; Michael, Nelson L; Krebs, Shelly J.
Affiliation
  • Sankhala RS; Emerging Infectious Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, MD, USA.
  • Lal KG; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA.
  • Jensen JL; Emerging Infectious Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, MD, USA.
  • Dussupt V; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA.
  • Mendez-Rivera L; Emerging Infectious Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, MD, USA.
  • Bai H; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA.
  • Wieczorek L; Emerging Infectious Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, MD, USA.
  • Mayer SV; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA.
  • Zemil M; U.S. Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD, USA.
  • Wagner DA; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA.
  • Townsley SM; U.S. Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD, USA.
  • Hajduczki A; Emerging Infectious Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, MD, USA.
  • Chang WC; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA.
  • Chen WH; U.S. Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD, USA.
  • Donofrio GC; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA.
  • Jian N; U.S. Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD, USA.
  • King HAD; Emerging Infectious Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, MD, USA.
  • Lorang CG; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA.
  • Martinez EJ; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA.
  • Rees PA; U.S. Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD, USA.
  • Peterson CE; Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA.
  • Schmidt F; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA.
  • Hart TJ; U.S. Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD, USA.
  • Duso DK; Emerging Infectious Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, MD, USA.
  • Kummer LW; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA.
  • Casey SP; Emerging Infectious Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, MD, USA.
  • Williams JK; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA.
  • Kannan S; Emerging Infectious Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, MD, USA.
  • Slike BM; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA.
  • Smith L; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA.
  • Swafford I; U.S. Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD, USA.
  • Thomas PV; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA.
  • Tran U; U.S. Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD, USA.
  • Currier JR; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA.
  • Bolton DL; U.S. Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD, USA.
  • Davidson E; Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA.
  • Doranz BJ; Emerging Infectious Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, MD, USA.
  • Hatziioannou T; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA.
  • Bieniasz PD; Emerging Infectious Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, MD, USA.
  • Paquin-Proulx D; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA.
  • Reiley WW; Emerging Infectious Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, MD, USA.
  • Rolland M; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA.
  • Sullivan NJ; Laboratory of Retrovirology, The Rockefeller University, New York, NY, USA.
  • Vasan S; Trudeau Institute, Saranac Lake, NY, USA.
  • Collins ND; Trudeau Institute, Saranac Lake, NY, USA.
  • Modjarrad K; Trudeau Institute, Saranac Lake, NY, USA.
  • Gromowski GD; Trudeau Institute, Saranac Lake, NY, USA.
  • Polonis VR; Integral Molecular, Philadelphia, PA, USA.
  • Michael NL; Integral Molecular, Philadelphia, PA, USA.
  • Krebs SJ; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA.
Nat Commun ; 15(1): 200, 2024 Jan 03.
Article in En | MEDLINE | ID: mdl-38172512
ABSTRACT
The repeat emergence of SARS-CoV-2 variants of concern (VoC) with decreased susceptibility to vaccine-elicited antibodies highlights the need to develop next-generation vaccine candidates that confer broad protection. Here we describe the antibody response induced by the SARS-CoV-2 Spike Ferritin Nanoparticle (SpFN) vaccine candidate adjuvanted with the Army Liposomal Formulation including QS21 (ALFQ) in non-human primates. By isolating and characterizing several monoclonal antibodies directed against the Spike Receptor Binding Domain (RBD), N-Terminal Domain (NTD), or the S2 Domain, we define the molecular recognition of vaccine-elicited cross-reactive monoclonal antibodies (mAbs) elicited by SpFN. We identify six neutralizing antibodies with broad sarbecovirus cross-reactivity that recapitulate serum polyclonal antibody responses. In particular, RBD mAb WRAIR-5001 binds to the conserved cryptic region with high affinity to sarbecovirus clades 1 and 2, including Omicron variants, while mAb WRAIR-5021 offers complete protection from B.1.617.2 (Delta) in a murine challenge study. Our data further highlight the ability of SpFN vaccination to stimulate cross-reactive B cells targeting conserved regions of the Spike with activity against SARS CoV-1 and SARS-CoV-2 variants.
Subject(s)
Fulltext
Print
XML
PubMed Links
Search on Google

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Severe acute respiratory syndrome-related coronavirus / Nanoparticles Limits: Animals Language: En Journal: Nat Commun Journal subject: BIOLOGIA / CIENCIA Year: 2024 Type: Article Affiliation country: United States
Fulltext
Print
XML
PubMed Links
Search on Google

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Severe acute respiratory syndrome-related coronavirus / Nanoparticles Limits: Animals Language: En Journal: Nat Commun Journal subject: BIOLOGIA / CIENCIA Year: 2024 Type: Article Affiliation country: United States