A Synergistic Combination of Oleanolic Acid and Apatinib to Enhance Antitumor Effect on Liver Cancer Cells and Protect against Hepatic Injury.
Recent Pat Anticancer Drug Discov
; 19(2): 199-208, 2024.
Article
in En
| MEDLINE
| ID: mdl-38214359
ABSTRACT
BACKGROUND:
As a pentacyclic triterpenoid, OA (oleanolic acid) has exhibited antiinflammatory, immunomodulatory and antitumor effects. VEGFR-2 (vascular endothelial cells receptor-2) tyrosine kinase activity could be inhibited by apatinib, a small-molecule antiangiogenic agent.OBJECTIVE:
Thus, this study sought to investigate the mechanism underlying the synergistic antitumor activity of combined OA and apatinib patent.METHODS:
Through CCK8 (Cell counting kit 8 assay), flow cytometric and western blotting techniques, we conducted in vitro studies on apatinib and OA effects on cell proliferation and apoptosis in H22 cell line. H22 tumor-burdened mice model was established in vivo, while the related signaling pathways were studied via pathological examination, western blotting and qPCR (quantitative polymerase chain reaction).RESULTS:
Growth of H22 cells in vitro and in vivo could be inhibited effectively by apatinib and OA. Thus, OA repaired liver function and inhibited oxidative stress induced by apatinib.CONCLUSION:
OA can treat apatinib induced liver injury in H22 Tumor-burdened mice by enhancing the suppresssive effect of apatinib on the growth of tumor.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Oleanolic Acid
/
Pyridines
/
Liver Neoplasms
Limits:
Animals
/
Humans
Language:
En
Journal:
Recent Pat Anticancer Drug Discov
Journal subject:
NEOPLASIAS
/
TERAPIA POR MEDICAMENTOS
Year:
2024
Type:
Article
Affiliation country:
China