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Effects of exposure to the neonicotinoid pesticide clothianidin on α-defensin secretion and gut microbiota in mice.
Yonoichi, Sakura; Hara, Yukako; Ishida, Yuya; Shoda, Asuka; Kimura, Mako; Murata, Midori; Nunobiki, Sarika; Ito, Makiko; Yoshimoto, Ayano; Mantani, Youhei; Yokoyama, Toshifumi; Hirano, Tetsushi; Ikenaka, Yoshinori; Yokoi, Yuki; Ayabe, Tokiyoshi; Nakamura, Kiminori; Hoshi, Nobuhiko.
Affiliation
  • Yonoichi S; Laboratory of Animal Molecular Morphology, Department of Animal Science, Graduate School of Agricultural Science, Kobe University, Hyogo, Japan.
  • Hara Y; Laboratory of Animal Molecular Morphology, Department of Animal Science, Graduate School of Agricultural Science, Kobe University, Hyogo, Japan.
  • Ishida Y; Laboratory of Animal Molecular Morphology, Department of Animal Science, Graduate School of Agricultural Science, Kobe University, Hyogo, Japan.
  • Shoda A; Laboratory of Animal Molecular Morphology, Department of Animal Science, Graduate School of Agricultural Science, Kobe University, Hyogo, Japan.
  • Kimura M; Laboratory of Animal Molecular Morphology, Department of Animal Science, Graduate School of Agricultural Science, Kobe University, Hyogo, Japan.
  • Murata M; Laboratory of Animal Molecular Morphology, Department of Animal Science, Graduate School of Agricultural Science, Kobe University, Hyogo, Japan.
  • Nunobiki S; Laboratory of Animal Molecular Morphology, Department of Animal Science, Graduate School of Agricultural Science, Kobe University, Hyogo, Japan.
  • Ito M; Laboratory of Animal Molecular Morphology, Department of Animal Science, Graduate School of Agricultural Science, Kobe University, Hyogo, Japan.
  • Yoshimoto A; Laboratory of Animal Molecular Morphology, Department of Animal Science, Graduate School of Agricultural Science, Kobe University, Hyogo, Japan.
  • Mantani Y; Laboratory of Histophysiology, Department of Animal Science, Graduate School of Agricultural Science, Kobe University, Hyogo, Japan.
  • Yokoyama T; Laboratory of Animal Molecular Morphology, Department of Animal Science, Graduate School of Agricultural Science, Kobe University, Hyogo, Japan.
  • Hirano T; Faculty of Pharmaceutical Sciences, University of Toyama, Toyama, Japan.
  • Ikenaka Y; Laboratory of Toxicology, Department of Environmental Veterinary Sciences, Faculty of Veterinary Medicine, Hokkaido University, Hokkaido, Japan.
  • Yokoi Y; Translational Research Unit, Veterinary Teaching Hospital, Faculty of Veterinary Medicine, Hokkaido University, Hokkaido, Japan.
  • Ayabe T; One Health Research Center, Hokkaido University, Hokkaido, Japan.
  • Nakamura K; Water Research Group, Unit for Environmental Sciences and Management, North-West University, Potchefstroom, South Africa.
  • Hoshi N; Innate Immunity Laboratory, Department of Cell Biological Science, Faculty of Advanced Life Science, Hokkaido University Graduate School of Life Science, Hokkaido, Japan.
J Vet Med Sci ; 86(3): 277-284, 2024 Mar 01.
Article in En | MEDLINE | ID: mdl-38267031
ABSTRACT
The mechanism by which the neonicotinoid pesticide clothianidin (CLO) disrupts the intestinal microbiota of experimental animals is unknown. We focused on α-defensins, which are regulators of the intestinal microbiota. Subchronic exposure to CLO induced dysbiosis and reduced short-chain fatty acid-producing bacteria in the intestinal microbiota of mice. Levels of cryptdin-1 (Crp1, a major α-defensin in mice) in feces and cecal contents were lower in the CLO-exposed groups than in control. In Crp1 immunostaining, Paneth cells in the jejunum and ileum of the no-observed-adverse-effect-level CLO-exposed group showed a stronger positive signal than control, likely due to the suppression of Crp1 release. Our results showed that CLO exposure suppresses α-defensin secretion from Paneth cells as part of the mechanism underlying CLO-induced dysbiosis.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Pesticides / Rodent Diseases / Thiazoles / Alpha-Defensins / Gastrointestinal Microbiome / Guanidines Limits: Animals Language: En Journal: J Vet Med Sci Journal subject: MEDICINA VETERINARIA Year: 2024 Type: Article Affiliation country: Japan
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Pesticides / Rodent Diseases / Thiazoles / Alpha-Defensins / Gastrointestinal Microbiome / Guanidines Limits: Animals Language: En Journal: J Vet Med Sci Journal subject: MEDICINA VETERINARIA Year: 2024 Type: Article Affiliation country: Japan