Deciphering Membrane-Protein Interactions and High-Throughput Antigen Identification with Cell Doublets.
Adv Sci (Weinh)
; 11(13): e2305750, 2024 Apr.
Article
in En
| MEDLINE
| ID: mdl-38342599
ABSTRACT
Deciphering cellular interactions is essential to both understand the mechanisms underlying a broad range of human diseases, but also to manipulate therapies targeting these diseases. Here, the formation of cell doublets resulting from specific membrane ligand-receptor interactions is discovered. Based on this phenomenon, the study developed DoubletSeeker, a novel high-throughput method for the reliable identification of ligand-receptor interactions. The study shows that DoubletSeeker can accurately identify T cell receptor (TCR)-antigen interactions with high sensitivity and specificity. Notably, DoubletSeeker effectively captured paired TCR-peptide major histocompatibility complex (pMHC) information during a highly complex library-on-library screening and successfully identified three mutant TCRs that specifically recognize the MART-1 epitope. In turn, DoubletSeeker can act as an antigen discovery platform that allows for the development of novel immunotherapy targets, making it valuable for investigating fundamental tumor immunology.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Receptors, Antigen, T-Cell
/
Antigens
Type of study:
Diagnostic_studies
Limits:
Humans
Language:
En
Journal:
Adv Sci (Weinh)
Year:
2024
Type:
Article
Affiliation country:
China