MiR-29a-3p mediates phosphatase and tensin homolog and inhibits osteoarthritis progression.
Funct Integr Genomics
; 24(2): 54, 2024 Mar 12.
Article
in En
| MEDLINE
| ID: mdl-38467932
ABSTRACT
Despite substantial progress in clinical trials of osteoarthritis (OA) gene therapy, the prevalence of OA is still on the rise. MiRNAs have a potential biomarker and therapeutic target for OA. OA cartilage and chondrosarcoma cells were studied to determine the role of miR-29a-3p and PTEN. OA cartilage and human chondrosarcoma cells (SW1353) were obtained. miR-29a-3p and PTEN signature expression was determined by RT-qPCR. The binding relationship between miR-29a-3p and PTEN was investigated by dual-luciferase reporter gene and western blot assay. TUNEL, immunohistochemistry, CCK-8, and flow cytometry were utilized to determine the proliferation and apoptosis of SW1353 cells. This study indicated downregulation of miR-29a-3p expression and upregulation of PTEN expression in human OA primary chondrocytes or OA tissue samples, compared with the normal cartilage cells or tissues. PTEN expression was negatively correlated with miR-29a-3p expression, and miR-29a-3p targeted PTEN mechanistically. miR-29a-3p reduced SW1353 cell activity and proliferation and promoted cell apoptosis. However, the aforementioned effects could be reversed by downregulating PTEN. miR-29a-3p can stimulate chondrocyte proliferation and inhibit apoptosis by inhibiting PTEN expression.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Osteoarthritis
/
Bone Neoplasms
/
Chondrosarcoma
/
MicroRNAs
Limits:
Humans
Language:
En
Journal:
Funct Integr Genomics
/
Funct. integr. geonomics (Internet)
/
Functional & integrative genomics (Internet)
Journal subject:
BIOLOGIA MOLECULAR
/
GENETICA
Year:
2024
Type:
Article
Affiliation country:
China