Association of genetic polymorphism of interleukin 1-alpha and interleukin 1-beta with external apical root resorption in orthodontic patients.
Int Orthod
; 22(2): 100869, 2024 Jun.
Article
in En
| MEDLINE
| ID: mdl-38513309
ABSTRACT
OBJECTIVE:
To assess the allele rs 1143634 in IL-1ß and rs1800587 in IL-1α in patients for orthodontically induced external apical root resorption (EARR). MATERIAL ANDMETHODS:
Intra-oral periapical radiograph (IOPA) of maxillary incisors of 142 Patients were evaluated for resorption at two time points; before the start of fixed mechanotherapy (T1) and after one year of treatment (T2). The individuals with root resorption<2mm were categorized as a control group (group 1; n=90), and resorption>2mm were categorized as case group (group 2; n=52). Buccal swabs of all patients were taken and DNA could be isolated in 95 out of 142 samples (group 1 {n=58}, group 2 {n=37}), which were then screened for the selected two polymorphic targets to determine the nucleotide status of these targets. Tetra-primer ARMS PCR reactions were carried out using all 4 primers for each polymorphism.RESULTS:
rs11800587 was not associated with risk of EARR in any inheritance model. Chi-square test for association of alleles with EARR revealed that rs1143634 was associated with the risk of EARR in an allelic model in such a way that A allele of this SNP increased the risk of EARR 4 folds [OR=4.375; P=0.016]. However, the adjusted level of significance using the Holm-Bonferroni method for rs1143634 was P<0.010 for A and G comparison rendering the results non-significant.CONCLUSION:
SNP rs1143634 and SNP rs11800587 were not associated with risk of EARR in any inheritance model.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Root Resorption
/
Polymorphism, Single Nucleotide
/
Interleukin-1alpha
/
Interleukin-1beta
Limits:
Adolescent
/
Adult
/
Child
/
Female
/
Humans
/
Male
Language:
En
Journal:
Int Orthod
/
Int. orthod
/
International orthodontics
Journal subject:
ORTODONTIA
Year:
2024
Type:
Article
Affiliation country:
India