Effect of levetiracetam on DNA oxidation and glutathione content in a temporal lobe epilepsy model.
Acta Neurobiol Exp (Wars)
; 84(1): 51-58, 2024 Mar 28.
Article
in En
| MEDLINE
| ID: mdl-38587324
ABSTRACT
Levetiracetam (LEV) is a drug commonly used as an anticonvulsant. However, recent evidence points to a possible role as an antioxidant. We previously demonstrated the antioxidant properties of LEV by significantly increasing catalase and superoxide dismutase activities and decreasing the hydrogen peroxide (H2O2) levels in the hippocampus of rats with temporal lobe epilepsy (TLE) showing scavenging properties against the hydroxyl radical. The aim of the present work was to evaluate, the effect of LEV on DNA oxidation, by determining 8hydroxy2deoxyguanosine (8OHdG) levels, and glutathione content, through reduced (GSH) and oxidized (GSSG) glutathione levels, in the hippocampus of rats with TLE. Male Wistar rats were assigned to the control (CTRL), CTRL+LEV, epileptic (EPI) and EPI+LEV groups. TLE was induced using the lithiumpilocarpine model. Thirteen weeks after TLE induction, LEV was administered for one week through osmotic pumps implanted subcutaneously. The determination of 8OHdG, GSH and GSSG levels were measured using spectrophotometric methods. We showed that LEV alone significantly increased 8OHdG and GSSG levels in the hippocampus of control rats compared to those in epileptic condition. No significant differences in GSH levels were observed. LEV could induce changes in the hippocampus increasing DNA oxidation and GSSG levels under nonepileptic condition but not protecting against the mitochondrial dysfunction observed in TLE probably by mechanisms related to changes in chromatin structure, neuroinflammation and alterations in redox components.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Piracetam
/
Epilepsy
/
Epilepsy, Temporal Lobe
Limits:
Animals
Language:
En
Journal:
Acta Neurobiol Exp (Wars)
/
Acta neurobiol. exp
/
Acta neurobiologiae experimentalis
Year:
2024
Type:
Article
Affiliation country:
Mexico