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The crucial role of stability of intercalating agent for DNA binding studies in DMSO/water system.
Bandyopadhyay, Krishanu; Verma, Abhineet; Pandey, Ankita; Walia, Rajat; Saha, Satyen.
Affiliation
  • Bandyopadhyay K; Department of Chemistry, Institute of Science, Banaras Hindu University, Varanasi 221005, India.
  • Verma A; Department of Chemistry, Institute of Science, Banaras Hindu University, Varanasi 221005, India.
  • Pandey A; Department of Chemistry, Institute of Science, Banaras Hindu University, Varanasi 221005, India.
  • Walia R; Department of Chemistry, City University of Hong Kong, Hong Kong SAR.
  • Saha S; Department of Chemistry, Institute of Science, Banaras Hindu University, Varanasi 221005, India. Electronic address: satyen.saha@gmail.com.
Spectrochim Acta A Mol Biomol Spectrosc ; 315: 124265, 2024 Jul 05.
Article in En | MEDLINE | ID: mdl-38626674
ABSTRACT
In recent years, extensive research has been directed towards understanding the interactions between various zinc complexes with DNA, specifically delving into their intercalation and binding behaviors. The binding of zinc complexes to DNA is particularly intriguing due to their distinctive intercalating capabilities. This study unveils a remarkable phenomenon observed with a specific Zn complex, ([B-Zn-N3], where B is a Schiff base ligand), during DNA intercalation investigations in the popular DMSO-Water binary solvent mixture. An unanticipated observation revealed time-dependent changes in the UV-visible absorption spectroscopic studies, coupled with the existence of an isosbestic point. This observation questions the stability of the intercalating agent itself during the intercalation process. The emergence of a decomposed product during the intercalation study has been confirmed through various analytical techniques, including CHN analysis, MALDI mass, XPS, Raman spectroscopy, and Powder XRD. The change in the chemical species on intercalation is further substantiated by theoretical studies, adding depth to our understanding of the intricate dynamics at play during DNA intercalation with the [B-Zn-N3] complex in the DMSO-Water system.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: DNA / Water / Dimethyl Sulfoxide / Intercalating Agents Language: En Journal: Spectrochim Acta A Mol Biomol Spectrosc Journal subject: BIOLOGIA MOLECULAR Year: 2024 Type: Article Affiliation country: India

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: DNA / Water / Dimethyl Sulfoxide / Intercalating Agents Language: En Journal: Spectrochim Acta A Mol Biomol Spectrosc Journal subject: BIOLOGIA MOLECULAR Year: 2024 Type: Article Affiliation country: India