Identification and functional characterization of interleukin-22 (IL-22) in orange-spotted grouper (Epinephelus coioides).
Fish Shellfish Immunol
; 150: 109598, 2024 Jul.
Article
in En
| MEDLINE
| ID: mdl-38697375
ABSTRACT
In mammals, IL-22 is considered as a critical cytokine regulating of immunity and homeostasis at barrier surfaces. Although IL-22 have been functional characterization in different species of fish, the studies about distinct responses of IL-22 in different organs/tissues/cell types is rather limited. Here, we identified and cloned IL-22 gene (named as Ec-IL-22) from grouper (Epinephelus coioides). Ec-IL-22 gene was detected in all orangs/tissues examined, and was induced in intestine, gill, spleen, head kidney, and primary head kidney/intestine leukocytes following the stimulation of LPS and poly (IC), as well as Vibrio harveyi and Singapore grouper iridovirus infection (SGIV). In addition, the stimulation of DSS could induce the expression of Ec-IL-22 in intestine and primary leukocytes from intestine. Importantly, the treatment of recombinant Ec-IL-22 induced the mRNA level of proinflammatory cytokines in primary intestine/head kidney leukocytes. The present results improve the understanding of expression patterns and functional characteristics of fish IL-22 in different organs/tissues/cell types.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Vibrio
/
Vibrio Infections
/
Bass
/
Gene Expression Regulation
/
Interleukins
/
Fish Proteins
/
DNA Virus Infections
/
Fish Diseases
/
Interleukin-22
Limits:
Animals
Language:
En
Journal:
Fish Shellfish Immunol
Journal subject:
BIOLOGIA
/
MEDICINA VETERINARIA
Year:
2024
Type:
Article