Dendritic cells as accessory cells in antigen-specific murine T lymphocyte proliferation in vitro.
J Immunol Methods
; 77(1): 131-8, 1985 Feb 28.
Article
in En
| MEDLINE
| ID: mdl-3871821
ABSTRACT
The role of dendritic cells in antigen-induced murine T lymphocyte activation was studied by addition of purified dendritic cells to purified lymph node T lymphocytes from ovalbumin-primed mice. In the presence of the priming antigen T cells generated an antigen-specific response. The response was at least 3-fold higher with the use of a modified IMDM culture medium. The complete requirement for accessory cells was demonstrated only when nylon wool-purified T lymphocytes were thoroughly depleted of Ia antigen-expressing cells. Dendritic cells as well as peritoneal exudate macrophages were equally effective as antigen-presenting cells.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Spleen
/
T-Lymphocytes
/
Antigen-Presenting Cells
Limits:
Animals
Language:
En
Journal:
J Immunol Methods
Year:
1985
Type:
Article