Influence factors of metagenomic next-generation sequencing negative results in diagnosed patients with spinal infection.
Diagn Microbiol Infect Dis
; 109(3): 116278, 2024 Jul.
Article
in En
| MEDLINE
| ID: mdl-38723451
ABSTRACT
The aim of this study was to evaluate the influence factors of metagenomic next-generation sequencing (mNGS) negative results in the diagnosed patients with spinal infection. mNGS test was applied in a cohort of 114 patients with suspected spinal infection, among which 56 patients had a final diagnosis of spinal infection. mNGS achieved a sensitivity of 75.0% (95% CI, 61.6% to 85.6%) and a specificity of 84.5% (95% CI, 72.6% to 92.7%), using histopathology and culture results as reference. Diagnosed patients with a negative culture result had lower white blood cell account, percentage of neutrophilic granulocyte, C-reactive protein (all P<0.05) and relatively higher rate of prior antimicrobial treatment history (P=0.059). However, diagnosed patients with a negative mNGS result did not have such difference with mNGS-positive patients, suggesting that mNGS was not strictly limited by the above indicators, which presented the advantages of this technique from another point of view.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Sensitivity and Specificity
/
Metagenomics
/
High-Throughput Nucleotide Sequencing
Limits:
Adult
/
Aged
/
Aged80
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Female
/
Humans
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Male
/
Middle aged
Language:
En
Journal:
Diagn Microbiol Infect Dis
Year:
2024
Type:
Article
Affiliation country:
China