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Using 16s rRNA sequencing to characterize the microbiome of tropical cutaneous ulcer disease: insights into the microbial landscape and implications for diagnosis and treatment.
Handley, Becca L; Sokana, Oliver; Addo, Kennedy Kwasi; Wagner, Josef; Fookes, María; Harding-Esch, Emma; Marks, Michael; Thomson, Nicholas R; Doyle, Ronan M.
Affiliation
  • Handley BL; Faculty of Infectious Diseases, London School of Hygiene and Tropical Medicine, London, UK.
  • Sokana O; Solomon Islands Ministry of Health, Honiara, Solomon Islands.
  • Addo KK; Noguchi Memorial Institute for Medical Research, University of Ghana, Accra, Ghana.
  • Wagner J; Wellcome Trust Sanger Institute, Cambridge, UK.
  • Fookes M; Wellcome Trust Sanger Institute, Cambridge, UK.
  • Harding-Esch E; Faculty of Infectious Diseases, London School of Hygiene and Tropical Medicine, London, UK.
  • Marks M; Faculty of Infectious Diseases, London School of Hygiene and Tropical Medicine, London, UK.
  • Thomson NR; Hospital for Tropical Diseases, London, UK.
  • Doyle RM; University College London, London, UK.
Microb Genom ; 10(5)2024 May.
Article in En | MEDLINE | ID: mdl-38739120
ABSTRACT
Cutaneous ulcers are common in yaws-endemic areas. Although often attributed to 'Treponema pallidum subsp. pertenue' and Haemophilus ducreyi, quantitative PCR has highlighted a significant proportion of these ulcers are negative for both pathogens and are considered idiopathic. This is a retrospective analysis utilising existing 16S rRNA sequencing data from two independent yaws studies that took place in Ghana and the Solomon Islands. We characterized bacterial diversity in 38 samples to identify potential causative agents for idiopathic cutaneous ulcers. We identified a diverse bacterial profile, including Arcanobacterium haemolyticum, Campylobacter concisus, Corynebacterium diphtheriae, Staphylococcus spp. and Streptococcus pyogenes, consistent with findings from previous cutaneous ulcer microbiome studies. No single bacterial species was universally present across all samples. The most prevalent bacterium, Campylobacter ureolyticus, appeared in 42% of samples, suggesting a multifactorial aetiology for cutaneous ulcers in yaws-endemic areas. This study emphasizes the need for a nuanced understanding of potential causative agents. The findings prompt further exploration into the intricate microbial interactions contributing to idiopathic yaw-like ulcers, guiding future research toward comprehensive diagnostic and therapeutic strategies.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Skin Ulcer / RNA, Ribosomal, 16S / Microbiota Limits: Adult / Female / Humans / Male / Middle aged Country/Region as subject: Africa Language: En Journal: Microb Genom Year: 2024 Type: Article Affiliation country: United kingdom

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Skin Ulcer / RNA, Ribosomal, 16S / Microbiota Limits: Adult / Female / Humans / Male / Middle aged Country/Region as subject: Africa Language: En Journal: Microb Genom Year: 2024 Type: Article Affiliation country: United kingdom