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A series of spontaneously blinking dyes for super-resolution microscopy.
Holland, Katie L; Plutkis, Sarah E; Daugird, Timothy A; Sau, Abhishek; Grimm, Jonathan B; English, Brian P; Zheng, Qinsi; Dave, Sandeep; Rahman, Fariha; Xie, Liangqi; Dong, Peng; Tkachuk, Ariana N; Brown, Timothy A; Singer, Robert H; Liu, Zhe; Galbraith, Catherine G; Musser, Siegfried M; Legant, Wesley R; Lavis, Luke D.
Affiliation
  • Holland KL; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.
  • Plutkis SE; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.
  • Daugird TA; Department of Pharmacology, University of North Carolina-Chapel Hill Medical School, Chapel Hill, NC, USA.
  • Sau A; Department of Cell Biology and Genetics, Texas A&M University, School of Medicine, College Station, TX, USA.
  • Grimm JB; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.
  • English BP; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.
  • Zheng Q; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.
  • Dave S; Department of Cell Biology and Genetics, Texas A&M University, School of Medicine, College Station, TX, USA.
  • Rahman F; Joint Department of Biomedical Engineering, University of North Carolina-Chapel Hill and North Carolina State University, Chapel Hill, NC, USA.
  • Xie L; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.
  • Dong P; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.
  • Tkachuk AN; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.
  • Brown TA; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.
  • Singer RH; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.
  • Liu Z; Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, New York, USA.
  • Galbraith CG; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.
  • Musser SM; Department of Biomedical Engineering and Knight Cancer Institute, Oregon Health and Science University, Portland, OR, USA.
  • Legant WR; Department of Cell Biology and Genetics, Texas A&M University, School of Medicine, College Station, TX, USA.
  • Lavis LD; Department of Pharmacology, University of North Carolina-Chapel Hill Medical School, Chapel Hill, NC, USA.
bioRxiv ; 2024 Feb 24.
Article in En | MEDLINE | ID: mdl-38766149
ABSTRACT
Spontaneously blinking fluorophores permit the detection and localization of individual molecules without reducing buffers or caging groups, thus simplifying single-molecule localization microscopy (SMLM). The intrinsic blinking properties of such dyes are dictated by molecular structure and modulated by environment, which can limit utility. We report a series of tuned spontaneously blinking dyes with duty cycles that span two orders of magnitude, allowing facile SMLM in cells and dense biomolecular structures.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Language: En Journal: BioRxiv Year: 2024 Type: Article Affiliation country: United States
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PubMed Links
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Full text: 1 Collection: 01-internacional Database: MEDLINE Language: En Journal: BioRxiv Year: 2024 Type: Article Affiliation country: United States