A series of spontaneously blinking dyes for super-resolution microscopy.
bioRxiv
; 2024 Feb 24.
Article
in En
| MEDLINE
| ID: mdl-38766149
ABSTRACT
Spontaneously blinking fluorophores permit the detection and localization of individual molecules without reducing buffers or caging groups, thus simplifying single-molecule localization microscopy (SMLM). The intrinsic blinking properties of such dyes are dictated by molecular structure and modulated by environment, which can limit utility. We report a series of tuned spontaneously blinking dyes with duty cycles that span two orders of magnitude, allowing facile SMLM in cells and dense biomolecular structures.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Language:
En
Journal:
BioRxiv
Year:
2024
Type:
Article
Affiliation country:
United States