Kitasatospora cathayae sp. nov., a novel endophytic actinomycete isolated from the leaves of Cathaya argyrophylla.
Int J Syst Evol Microbiol
; 74(5)2024 May.
Article
in En
| MEDLINE
| ID: mdl-38809239
ABSTRACT
Strain HUAS 3-15T was isolated from the leaves of Cathaya argyrophylla collected from Chenzhou, Hunan Province, PR China. The main fatty acids (>5.0â%) of the strain were anteiso-C15ââ0, C16ââ0, C18ââ1 ω9c, iso-C16ââ0, summed feature 5 (C18ââ2 ω6,9c/C18ââ0 ante), iso-C15ââ0 and anteiso-C17ââ0. MK-9(H6), MK-9(H8) and MK-9(H4) were detected as respiratory quinones. The diagnostic cell-wall diamino acid was meso-diaminopimelic acid. Galactose, glucose and ribose were also present in the cell wall. The major polar lipids consisted of diphosphatidylglycerol, phosphatidyl ethanolamine, phosphatidylinositol mannosides and unidentified phospholipids. The DNA G+C content of the genome sequence, consisting of 8â860â963 bp, is 72.4âmol%. blast analysis based on 16S rRNA gene sequences revealed that the strain belongs to the genus Kitasatospora, with 99.37, 99.03, 98.95, 98.68 and 98.67â% sequence similarity to Kitasatospora aureofaciens ATCC 10762T, Kitasatospora viridis DSM 44826T, Kitasatospora xanthocidica NBRC 13469T, Kitasatospora aburaviensis NRRL B-2218T and Kitasatospora kifunensis IFO 15206T, respectively. Phylogenetic trees based on 16S rRNA gene and whole-genome sequences demonstrated that strain HUAS 3-15T formed a well-supported cluster with K. aureofaciens ATCC 10762T. Further genomic characterization through average nucleotide identity (ANIb/m) and digital DNA-DNA hybridization analysis between strain HUAS 3-15T and K. aureofaciens ATCC 10762T showed values of 90.62/92.55â% and 45.3â%, respectively, lower than the 95-96â% ANI threshold and 70.0â% cutoff used as guideline values for species delineation in bacteria. Furthermore, the differences between the strain and its phylogenomic neighbour in terms of physiological (e.g. sole carbon source growth) and chemotaxonomic (e.g. cellular fatty composition) characteristics further supported this conclusion. Consequently, we concluded that strain HUAS 3-15T represents a novel species of the genus Kitasatospora, for which the name Kitasatospora cathayae sp. nov. is proposed. The type strain is HUAS 3-15T (=MCCC 1K08542T=JCM 36274T).
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Phospholipids
/
Phylogeny
/
Base Composition
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DNA, Bacterial
/
RNA, Ribosomal, 16S
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Bacterial Typing Techniques
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Sequence Analysis, DNA
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Plant Leaves
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Fatty Acids
/
Endophytes
Country/Region as subject:
Asia
Language:
En
Journal:
Int J Syst Evol Microbiol
Journal subject:
MICROBIOLOGIA
Year:
2024
Type:
Article