Immunodeviation towards T cell-mediated immune response in the testes of LPS-induced mouse epididymo-orchitis.
J Reprod Immunol
; 164: 104272, 2024 Aug.
Article
in En
| MEDLINE
| ID: mdl-38838578
ABSTRACT
The testicular consequences of acute epididymo-orchitis remain largely unelucidated in long-term damage, which might be a neglected factor for male infertility. In this study, the differential phenotype of testicular immune cell subpopulations in lipopolysaccharide (LPS)-induced mouse epididymo-orchitis were analyzed by flow cytometry on day 1, day 7, and day 28. The number of macrophages, neutrophils, and myeloid-derived suppressor cells (MDSCs) steadily decreased in the testes with inoculation. Total F4/80-CD11c+ dendritic cells (DCs) maintained a relatively stable level, whereas conventional type 1 dendritic cells (cDC1) increased gradually from day 1 to day 28. There was a lower number of CD4+ and CD8+ T cells at day 1 and day 7, and they had similar results with a ceiling level at day 28. The testes displayed a higher level of CD3+ T cells but a lower frequency of macrophages, cDC2, and neutrophils at 28 days post-inoculation compared with the epididymis. In summary, our data indicates acute epididymo-orchitis could lead to long-term damage in the testes, which is characterized by CD3+ T cell (including CD4+ and CD8+ T cells)-mediated immune responses.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Orchitis
/
Testis
/
Lipopolysaccharides
/
Epididymitis
Limits:
Animals
/
Humans
/
Male
Language:
En
Journal:
J Reprod Immunol
Year:
2024
Type:
Article
Affiliation country:
China