Spatial Proteomics of Single Hepatocytes with Multiplexed Data-Independent Acquisition (mDIA).
Methods Mol Biol
; 2817: 97-113, 2024.
Article
in En
| MEDLINE
| ID: mdl-38907150
ABSTRACT
Spatially resolved mass spectrometry-based proteomics at single-cell resolution promises to provide insights into biological heterogeneity. We describe a protocol based on multiplexed data-independent acquisition (mDIA) with dimethyl labeling to enhance proteome depth, accuracy, and throughput while minimizing costs. It enables high-quality proteome analysis of single isolated hepatocytes and utilizes liver zonation for single-cell proteomics benchmarking. This adaptable, modular protocol will promote the use of single-cell proteomics in spatial biology.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Proteome
/
Hepatocytes
/
Proteomics
/
Single-Cell Analysis
Limits:
Animals
Language:
En
Journal:
Methods Mol Biol
/
Methods in molecular biology
/
Methods mol. biol
Journal subject:
BIOLOGIA MOLECULAR
Year:
2024
Type:
Article
Affiliation country:
Germany