The cytokine response of human coronary artery endothelial cells treated with doxorubicin: results of an in vitro experiment.
Biomed Khim
; 70(3): 156-160, 2024 Jun.
Article
in En
| MEDLINE
| ID: mdl-38940204
ABSTRACT
The cytokine profile of primary coronary artery endothelial cells cultivated in the presence of doxorubicin (2 µg/ml and 6 µg/ml) was evaluated using enzyme-linked immunosorbent assay and qPCR. Cultivation of cells in the presence of these concentrations of doxorubicin for 24 h, upregulated expression of the following genes IL6 (by 2.30 and 2.66 times, respectively), IL1B (by 1.25 and 3.44 times), and CXCL8 (by 6.47 times and 6.42 times), MIF (2.34 and 2.28 times), CCL2 (4.22 and 3.98 times). Under these conditions the following genes were downregulated IL10, IL1R2, TNF. Cultivation of cells in the presence of doxorubicin (2 µg/ml and 6 µg/ml) for 24 h also increased the secretion of IL-6.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Doxorubicin
/
Interleukin-6
/
Coronary Vessels
/
Endothelial Cells
Limits:
Humans
Language:
En
Journal:
Biomed Khim
Year:
2024
Type:
Article
Affiliation country:
RUSSIA