Single-nucleus RNA sequencing reveals glial cell type-specific responses to ischemic stroke in male rodents.
Nat Commun
; 15(1): 6232, 2024 Jul 24.
Article
in En
| MEDLINE
| ID: mdl-39043661
ABSTRACT
Neuroglia critically shape the brain´s response to ischemic stroke. However, their phenotypic heterogeneity impedes a holistic understanding of the cellular composition of the early ischemic lesion. Here we present a single cell resolution transcriptomics dataset of the brain´s acute response to infarction. Oligodendrocyte lineage cells and astrocytes range among the most transcriptionally perturbed populations and exhibit infarction- and subtype-specific molecular signatures. Specifically, we find infarction restricted proliferating oligodendrocyte precursor cells (OPCs), mature oligodendrocytes and reactive astrocytes, exhibiting transcriptional commonalities in response to ischemic injury. OPCs and reactive astrocytes are involved in a shared immuno-glial cross talk with stroke-specific myeloid cells. Within the perilesional zone, osteopontin positive myeloid cells accumulate in close proximity to CD44+ proliferating OPCs and reactive astrocytes. In vitro, osteopontin increases the migratory capacity of OPCs. Collectively, our study highlights molecular cross talk events which might govern the cellular composition of acutely infarcted brain tissue.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Oligodendroglia
/
Astrocytes
/
Single-Cell Analysis
/
Oligodendrocyte Precursor Cells
/
Ischemic Stroke
Limits:
Animals
Language:
En
Journal:
Nat Commun
/
Nature communications
Journal subject:
BIOLOGIA
/
CIENCIA
Year:
2024
Type:
Article
Affiliation country:
Austria