A multi-nutrient array protocol to study disease-diet interactions in Drosophila melanogaster.
STAR Protoc
; 5(3): 103225, 2024 Sep 20.
Article
in En
| MEDLINE
| ID: mdl-39083384
ABSTRACT
The availability of a defined fully synthetic diet for the fruit fly Drosophila melanogaster allows for complete and precise manipulation of its nutritional environment. Here, we present a protocol for performing large-scale multivariate nutrient analysis via the traditional diet preparation approach, or by adding nutrient solutions to a baseline medium. We detail procedures from sample collection to data analysis. This protocol has applications for the study of nutrition-life trait interactions and nutrigenomics, to reveal interactions between genotype and diet composition. For complete details on the use and execution of this protocol, please refer to Martelli et al.1 and Martelli et al.2.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Diet
/
Drosophila melanogaster
Limits:
Animals
Language:
En
Journal:
STAR Protoc
Year:
2024
Type:
Article
Affiliation country:
Australia