Preferential binding of adriamycin and nogalamycin to DNase-I hypersensitive sites of Sarcoma-180 chromatin.
Chem Biol Interact
; 57(1): 65-72, 1986 Jan.
Article
in En
| MEDLINE
| ID: mdl-3948285
ABSTRACT
Binding of nogalamycin and adriamycin with Sarcoma-180 ascites tumor cell chromatin was studied by a spectrofluorometric method. There was significant reduction in the number of available drug binding sites per nucleotide when the chromatin was digested with DNase I for a period which releases only 7% of the chromosomal DNA. Results indicate preferential binding of these drugs with DNase I hypersensitive sites of chromatin. The DNase-I hypersensitive sites of chromatin were shown to correlate to the sequences required for gene expression. Further digestion with DNase I increases availability of drug binding sites, probably due to relaxation of the compact chromatin.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Sarcoma 180
/
Chromatin
/
Daunorubicin
/
Doxorubicin
/
Nogalamycin
/
Deoxyribonuclease I
Limits:
Animals
Language:
En
Journal:
Chem Biol Interact
Year:
1986
Type:
Article