DNA polymerases from RNA tumor viruses and human cells: inhibition by polyuridylic acid.

Abrell, J W; Smith, R G; Robert, M S; Gallo, B C

Abrell, J W; Smith, R G; Robert, M S; Gallo, B C.

Science ; 177(4054): 1111-4, 1972 Sep 22.

Article in En | MEDLINE | ID: mdl-4115520

ABSTRACT

ABSTRACT

Polyuridylic acid inhibited DNA polymerases purified from three species of oncornaviruses as well as three out of seven DNA polymerases purified from cells. Viral and cellular DNA polymerases could not be distinguished by polyuridylic acid inhibition, but were easily distinguished by their template preferences in the presence of magnesium.

Subject(s)

DNA Nucleotidyltransferases/antagonists & inhibitors; Oncogenic Viruses/enzymology; Polynucleotides/pharmacology; RNA Viruses/enzymology; Uracil Nucleotides/pharmacology; Animals; Avian Leukosis Virus/enzymology; DNA/metabolism; DNA Nucleotidyltransferases/isolation & purification; DNA Nucleotidyltransferases/metabolism; Escherichia coli/enzymology; Haplorhini; Humans; Kinetics; Leukemia, Lymphoid/enzymology; Lymphocytes/enzymology; Mice; Polynucleotides/metabolism; RNA-Directed DNA Polymerase/isolation & purification; RNA-Directed DNA Polymerase/metabolism; Rauscher Virus/enzymology; Reverse Transcriptase Inhibitors; Templates, Genetic

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Collection: 01-internacional Database: MEDLINE Main subject: Oncogenic Viruses / Polynucleotides / RNA Viruses / Uracil Nucleotides / DNA Nucleotidyltransferases Limits: Animals / Humans Language: En Journal: Science Year: 1972 Type: Article

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