Specific binding activity of isolated light chains of antibodies.
Science
; 157(3789): 707-9, 1967 Aug 11.
Article
in En
| MEDLINE
| ID: mdl-6028046
ABSTRACT
Free light chains isolated from specifically purified antibody have been shown to bind specific hapten. This proves that part of the binding site does exist on the light chain. The light chains were obtained from antibody directed against the 4-azonaphthalene-1-sulfonate group, and the binding of the simple hapten 4-anilinonaphthalene-1-sulfonate was determined by the fluorescence-enhancement technique. Since this hapten undergoes a striking increase in fluorescence on binding to light chains (and also on binding to specific antibody), the presence of small amounts of bound hapten could be determined, even in the presence of the high concentrations of unbound hapten required because of the low binding constant.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Peptides
/
Haptens
/
Antibodies
/
Antigen-Antibody Reactions
Language:
En
Journal:
Science
Year:
1967
Type:
Article