In vitro catenation and decatenation of DNA and a novel eucaryotic ATP-dependent topoisomerase.
Cell
; 20(2): 461-7, 1980 Jun.
Article
in En
| MEDLINE
| ID: mdl-6248247
ABSTRACT
Extracts from X. laevis germinal vesicles interlock duplex DNA circles to form catenanes. The catenation activity requires Mg++ and ATP. Negatively supercoiled or relaxed DNA can be used as substrates for the catenation reaction. Homology between donor and acceptor DNA is not required, since catenanes are formed between DNA molecules with unrelated sequences. In the course of the isolation of the activity responsible for the catenation reaction, we discovered a new ATP-dependent topoisomerase. The fractions containing the novel topoisomerase catenate and decatenate DNA, the ionic strength dictating which of the two opposing reactions will occur.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Oocytes
/
Ovum
/
DNA, Circular
/
DNA Topoisomerases, Type I
Limits:
Animals
Language:
En
Journal:
Cell
Year:
1980
Type:
Article