Sequence homology of Chinese hamster metallothionein genes I and II to those of the mouse and rat, and their amplification in Cd-resistant cells.
Biochim Biophys Acta
; 1219(3): 581-91, 1994 Nov 22.
Article
in En
| MEDLINE
| ID: mdl-7948015
ABSTRACT
The metallothionein (MT) I and II genes were isolated from Chinese hamster cells and sequenced. The MT-II gene is located about 6 kb upstream of the MT-I gene and their arrangement is similar to those of the mouse and rat MT genes. The sequence of the Chinese hamster MT-I gene is highly homologous to those of the mouse and rat, particularly in their promoter regions of MT-I. However, the promoter region of MT-II has less homology with those of the mouse and rat due t to insertions and deletions. The MT-I and MT-II genes were equally amplified 4-8-times in the Cd-resistant Chinese hamster cells, suggesting that both genes are included in the same amplification unit. Cytogenetic analysis of Cd-resistant cells by in situ hybridization showed that they are randomly integrated into multiple sites on the chromosomes.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Cadmium
/
Gene Amplification
/
Metallothionein
/
Muridae
Limits:
Animals
Language:
En
Journal:
Biochim Biophys Acta
Year:
1994
Type:
Article
Affiliation country:
Japan