Resolution and sensitivity enhancement of heteronuclear correlation for methylene resonances via 2H enrichment and decoupling.
J Biomol NMR
; 3(6): 701-8, 1993 Nov.
Article
in En
| MEDLINE
| ID: mdl-8111233
ABSTRACT
Monodeuterated methylene positions exhibit substantially superior spectral characteristics in 1H-13C correlation experiments as compared to diprotio signals. A combination of 2H decoupling and multiplet editing of HMQC and HSQC experiments provides resolution enhancement for both stereoselective and random fractionally deuterated samples. For HMQC experiments with [2-2HR,2-13C]glycine-labeled E. coli thioredoxin (11.7 kDa), 3-fold increases in both 1H and 13C resolution result in a complementary 9-fold enhancement in sensitivity. Owing to a smaller improvement in 13C resolution, the corresponding enhancements for the HSQC experiment are 2-fold less.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Hydrogen
/
Methane
Type of study:
Diagnostic_studies
Language:
En
Journal:
J Biomol NMR
Journal subject:
BIOLOGIA MOLECULAR
/
DIAGNOSTICO POR IMAGEM
/
MEDICINA NUCLEAR
Year:
1993
Type:
Article