Immunoenzymatic detection of staphylococcal enterotoxins: international interlaboratory study.
J AOAC Int
; 79(5): 1095-101, 1996.
Article
in En
| MEDLINE
| ID: mdl-8823918
ABSTRACT
An international interlaboratory study was performed by 13 laboratories to validate a commercially available, rapid enzyme immunoassay for detection of staphylococcal enterotoxins A, B, C, D, and E in foods. The 5 enterotoxin serotypes were detected at a level of 0.5 ng/g in mushrooms and ravioli, 0.8 ng/g in meat, 1 ng/mL in milk, and 1.5 ng/g in raw milk cheese when these foods were artificially contaminated with enterotoxin A. Enterotoxins A, B, C, D, or E were also detected in culture supernatants with no protein A interference when normal rabbit serum was used. This method was validated by the French Normalization Agency for the identification of staphylococcal enterotoxins in foods and culture fluids.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Staphylococcal Food Poisoning
/
Staphylococcus aureus
/
Enterotoxins
Type of study:
Clinical_trials
/
Diagnostic_studies
/
Prognostic_studies
Language:
En
Journal:
J AOAC Int
Year:
1996
Type:
Article
Affiliation country:
France