Isoflurane increases the uptake of glutamate in synaptosomes from rat cerebral cortex.
Br J Anaesth
; 78(1): 55-9, 1997 Jan.
Article
in En
| MEDLINE
| ID: mdl-9059205
ABSTRACT
We have studied the effect of increasing concentrations of isoflurane on high- and low-affinity uptake of L-glutamate using synaptosomes from rat cerebral cortex. In the high-affinity uptake range, 0.5% isoflurane had no effect on uptake velocity, while 1.5% and 3.0% isoflurane caused an increase in mean Vmax to 131 (SEM 54) and 210 (103)% of control, respectively. There was no significant change in the K(m) value. Vmax and K(m) values for low-affinity uptake of L-glutamate were unchanged by 1.5% isoflurane. These results provide evidence for an isoflurane-induced increase in high-affinity uptake of glutamate into presynaptic terminals. This effect may contribute to a reduction of transmitter in the synaptic cleft and thereby decreased excitatory synaptic transmission.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Synaptosomes
/
Cerebral Cortex
/
Glutamic Acid
/
Anesthetics, Inhalation
/
Isoflurane
Limits:
Animals
Language:
En
Journal:
Br J Anaesth
Year:
1997
Type:
Article
Affiliation country:
Norway