Up-regulation of transcription factors GATA-1 and GATA-2 induced by Panax notoginosides in hematopoietic cells / 中华血液学杂志
Chinese Journal of Hematology
; (12): 281-284, 2004.
Article
in Zh
| WPRIM
| ID: wpr-291448
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To observe the role of Panax notoginosides (PNS) in up-regulation of GATA family transcription factors, and explore intracellular signal pathway of PNS in the proliferation of hematopoietic cells.</p><p><b>METHODS</b>Human bone marrow cells were incubated with different concentrations of PNS for colony-forming assay. Human cell lines HL-60, K562, CHRF-288 and Meg-01 were incubated with PNS (10 mg/L) for 14 days. The cell nuclear proteins were extracted and analyzed by Western blot with antibodies against GATA-1, GATA-2. Electrophoretic mobility shift assay (EMSA) and antibody gel supershift assay was performed using (32)P labeled GATA consensus oligonucleotide which contains binding site for GATA transcription factors.</p><p><b>RESULTS</b>PNS could promote the proliferation of CFU-GM and CFU-E and induce the expression of GATA-1, GATA-2. The nuclear proteins of both GATA-1 and GATA-2 in K562, CHRF-288 and Meg-01 cells treated by PNS were increased by (1.5 - 2.8) and (2.0 - 3.1)-fold over untreated cells respectively. GATA binding activity initiated by PNS was apparently elevated to form higher density band of GATA-DNA complex. While there was no detectable change in HL-60 cells before and after PNS treatment. The predominant GATA binding complex was mainly attributable to both GATA-1 and GATA-2 proteins being in phosphorylated status.</p><p><b>CONCLUSION</b>PNS can induce the synthesis of transcription factors GATA-1 and GATA-2 and enhance their DNA binding activity, which could play a role in the up-regulation of the expression genes related to proliferation and differentiation in hematopoietic cells.</p>
Full text:
1
Database:
WPRIM
Main subject:
Pharmacology
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Bone Marrow Cells
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Up-Regulation
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Chemistry
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Blotting, Western
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HL-60 Cells
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K562 Cells
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Ginsenosides
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Cell Line, Tumor
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Cell Proliferation
Limits:
Humans
Language:
Zh
Journal:
Chinese Journal of Hematology
Year:
2004
Type:
Article