Effects of RhoA gene silencing by RNA interference on invasion of tongue carcinoma / 华西口腔医学杂志
West China Journal of Stomatology
; (6): 183-188, 2016.
Article
in Zh
| WPRIM
| ID: wpr-309157
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the effects of RhoA down-regulation by RNA interference on the invasion of tongue carcinoma Tca8113 and SCC-4.</p><p><b>METHODS</b>Determination of the human RhoA sequence as well as the design and constructionof a short specific small interfering RNAs (siRNA) were performed. The siRNA of RhoA gene was transfected into humantongue squamous cell carcinoma Tca8113 and SCC-4 cells line by Lipofectamine 2000. Quantitative real-time polymerasechain reaction was used to examine the mRNA expressionlevels of RhoA. Protein expressions of mRNA, galectin-3,and matrix metalloproteinase (MMP)-9 were evaluated byWestern blot. Transwell invasion assay was performed toassess the invasion ability of tongue carcinoma.</p><p><b>RESULTS</b>RhoA expressions in Tca8113 and SCC-4 cells were reducedsignificantly after transfection of RhoA-siRNA. Protein levels f galectin-3 and MVP-9 were also down-regulated significantly. Invasion ability was inhibited as well.</p><p><b>CONCLUSION</b>RhoA-siRNA can effectively inhibit RhoA expression in Tca8113 and SCC-4 cells. The invasion ability of tongue carcinoma cells decreased with down-regulation of the protein expressions of galectin-3 and MMP-9, indicating that RhoA-siRNA can inhibit invasion of tongue carcinoma. Results show that RhoA may play an important role in the processes of invasion and metastasis of tongue carcinoma.</p>
Full text:
1
Database:
WPRIM
Main subject:
Pathology
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RNA, Messenger
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Tongue Neoplasms
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Carcinoma, Squamous Cell
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Transfection
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Down-Regulation
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Matrix Metalloproteinase 9
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Gene Silencing
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Galectin 3
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RNA, Small Interfering
Limits:
Humans
Language:
Zh
Journal:
West China Journal of Stomatology
Year:
2016
Type:
Article