Effect of hepatocyte growth factor gene transfection on biological features of lymphoma cells / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
; (6): 378-385, 2010.
Article
in Zh
| WPRIM
| ID: wpr-319891
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the biological effect of hepatocyte growth factor (HGF) on HGF gene-transfected Raji cells.</p><p><b>METHODS</b>Total RNA was extracted from human hepatic tissue, HGF gene cDNA was amplified by RT-PCR, and then cloned into vector pVITRO2-mcs to construct recombinant eukaryotic expression vector pVITRO2-mcs-HGF. The recombinant vector was transfected to Raji cells, and the stably transfected cells were selected by homomycin B in serial passages, and confirmed by real-time fluorescent quantitative PCR, ELISA, immunocytohistochemistry. The biological features of transfected Raji cells were evaluated by semisolid culture.</p><p><b>RESULTS</b>RT-PCR results showed that Raji cells were transfected successfully with recombinant eukaryotic expression vector pVITRO2-mcs-HGF. HGF mRNA and protein were expressed successfully in Raji cells. Expression of HGF gene enhanced proliferation, metastasis and invasion of Raji cells.</p><p><b>CONCLUSION</b>HGF gene has been cloned and recombined to construct recombinant eukaryotic expression vector pVITRO2-mcs-HGF successfully. Transfected HGF may change the biological features of Raji cells.</p>
Full text:
1
Database:
WPRIM
Main subject:
Pathology
/
Recombinant Proteins
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RNA, Messenger
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Transfection
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Lymphoma, B-Cell
/
Cloning, Molecular
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Hepatocyte Growth Factor
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Cell Line, Tumor
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Genetics
Limits:
Humans
Language:
Zh
Journal:
Journal of Zhejiang University. Medical sciences
Year:
2010
Type:
Article