Protective effect of resveratrol against oxidative damage of UVA irradiated HaCaT cells / 中南大学学报(医学版)

ABSTRACT

Objective To observe the photoprotective effect and possible mechanisms of resveratrol for ultraviolet A (UVA) irradiated HaCaT cells. Methods HaCaT cells under UVA irradiation with 5 J/cm2 were interfered with 0.01 mmol/L and 0.1 mmol/L resveratrol. The testing objects were divided into a control and a UVA irradiation group, and then we detected the proliferation capacity with methylthiazdyl tetrazolium (MTT) and superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) activity, content of maleic dialdehyde (MDA) with hydroxylamine, colorimetric, thiobarbituric acid (TBA) methods. The ultrastructure was observed under electron microscope. Results Resveratrol could enhance the proliferation activity, SOD, GSH-Px activity of HaCaT cells under UVA irradiation, decrease the content of MDA in dose-dependent manner (P＜0.05). The electron microscope revealed that resveratrol could relieve the injury of HaCaT cells'ultrastructure. Conclusion Resveratrol can relieve the inhibition to HaCaT cell proliferation, injury of their ultrastructure and oxidation by UVA irradiation. The protection is dose-dependent. That resveratrol raises the oxidase activity and clears the oxyradical may account for these results.