Establishment and biological characteristics of a new human endometrial carcinoma / 中国病理生理杂志
Chinese Journal of Pathophysiology
; (12)1989.
Article
in Zh
| WPRIM
| ID: wpr-524895
Responsible library:
WPRO
ABSTRACT
AIM:
To establish a new human endometial carcinoma cell line for basic and clinical study.METHODS:
The minced tumor tissues were inoculated subcutaneously into the back of nude mice. After forming solid tumor, the mice were killed and the transplant was taken out for primery culture.RESULTS:
The established cell line was maintained by serial passages, named EAC. EAC cell line grew rapidly,steadily, and it had similar microscopic morphology to adenocarcinoma cell. Chromosome analysis reveal EAC chromosome counts ranging in hyperdiploid. The expression of ER, PR, P53, MDM2 were negative by SP immunohistochemistry. Tumorigenicity of EAC in nude mice was 100%.CONCLUSION:
A new human endometrial carcinoma, EAC, is established successfully, which is helpful for the basic and clinical study of endometrial carcinoma.
Full text:
1
Database:
WPRIM
Language:
Zh
Journal:
Chinese Journal of Pathophysiology
Year:
1989
Type:
Article