Design of recombinase and terminator-based genetic switches for cell state control / 生物工程学报
Chinese Journal of Biotechnology
; (12): 1874-1885, 2018.
Article
in Zh
| WPRIM
| ID: wpr-776281
Responsible library:
WPRO
ABSTRACT
Various genetic switches have been developed to let engineered cells perform designed functions. However, a sustained input is often needed to maintain the on/off state, which is energy-consuming and sensitive to perturbation. Therefore, we developed a set of transcriptional switches for cell states control that were constructed by the inversion effect of site-specific recombinases on terminators. Such a switch could respond to a pulse signal and maintain the new state by itself until the next input. With a bottom-up design principle, we first characterized the terminators and recombinases. Then the mutual interference was studied to select compatible pairs, which were used to achieve one-time and two-time state transitions. Finally, we constructed a biological seven-segment display as a demonstration to prove such switch's immense potential for application.
Key words
Full text:
1
Database:
WPRIM
Main subject:
Recombinases
/
Metabolism
Language:
Zh
Journal:
Chinese Journal of Biotechnology
Year:
2018
Type:
Article