Selection for transgene homozygosity in embryonic stem cells results in extensive loss of heterozygosity.
Nat Genet
; 27(3): 257-8, 2001 Mar.
Article
en En
| MEDLINE
| ID: mdl-11242104
ABSTRACT
Embryonic stem cells offer unprecedented opportunities for random or targeted genome alterations in the mouse. We present here an efficient strategy to create chromosome-specific loss of heterozygosity in embryonic stem cells. The combination of this method with genome-wide mutagenesis in ES cells (using chemical mutagens or gene-trap vectors) opens up the possibility for in vitro or in vivo functional screening of recessive mutations in the mouse.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Células Madre
/
Pérdida de Heterocigocidad
Límite:
Animals
Idioma:
En
Revista:
Nat Genet
Asunto de la revista:
GENETICA MEDICA
Año:
2001
Tipo del documento:
Article
País de afiliación:
Canadá