Complex formation of double-stranded DNA (6-4) photoproducts and anti-(6-4) photoproduct antibody Fabs.
Nucleic Acids Symp Ser
; (44): 151-2, 2000.
Article
en En
| MEDLINE
| ID: mdl-12903313
ABSTRACT
DNA (6-4) photoproducts are major constituents of ultraviolet-damaged DNAs. We prepared double-stranded (ds) (6-4) DNA photoproducts and analyzed formation of their complexes with anti-(6-4) photoproduct antibody Fabs. Elution profiles of the mixtures of ds-(6-4) DNAs and Fabs from anion-exchange and gel-filtration columns indicate that Fab 64M-2 deprives 14mer ds-(6-4) DNA of single-stranded (ss) (6-4) DNA and shows no interaction with 18 mer ds-(6-4) DNA (A18). Fab 64M-5 with an approximately 100-fold higher affinity than Fab 64M-2 forms a complex with the ds-(6-4) DNA (A18), but partly dissociates another 18 mer ds-(6-4) DNA (A18-3), with a lowered G-C content, into ss-DNAs. From these results, antibody 64M-5 possibly accommodates the T(6-4)T photolesion moiety of the ds-(6-4) DNA (A18) by flipping out the moiety from its neighboring segments.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
ADN
/
Fragmentos Fab de Inmunoglobulinas
Límite:
Animals
Idioma:
En
Revista:
Nucleic Acids Symp Ser
Año:
2000
Tipo del documento:
Article
País de afiliación:
Japón