Characterizing monoclonal antibody epitopes by filtered gene fragment phage display.
Biochem J
; 388(Pt 3): 889-94, 2005 Jun 15.
Article
en En
| MEDLINE
| ID: mdl-15720292
ABSTRACT
In the present paper, we describe a novel approach to map monoclonal antibody epitopes, using three new monoclonal antibodies that recognize h-TG2 (human transglutaminase 2) as an example. The target gene was fragmented and cloned upstream of an antibiotic-resistance gene, in the vector pPAO2, to select for in-frame polypeptides. After removal of the antibiotic-resistance gene by Cre/Lox recombination, an antigen fragment phage display library was created and selected against specific monoclonal antibodies. Using the h-TG2 fragment library, we were able to identify epitopes. This technique can also be broadly applied to the study of protein-protein interactions.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Mapeo Epitopo
/
Biblioteca de Péptidos
/
Anticuerpos Monoclonales
/
Epítopos
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Biochem J
Año:
2005
Tipo del documento:
Article
País de afiliación:
Italia