Grouping of streptomycetes using 16S-ITS RFLP fingerprinting.
Res Microbiol
; 156(5-6): 755-62, 2005.
Article
en En
| MEDLINE
| ID: mdl-15950131
ABSTRACT
A total of 463 Streptomyces and Kitasatospora type strains were screened using 16S-ITS RFLP fingerprinting (combined restriction digest using enzymes BstUI and HaeIII). In total, 59 clusters could be delineated, each comprising multiple strains with nearly identical patterns. Good correlation was found in general with phylogeny, as revealed by 16S rDNA sequencing. Most strains assigned to a particular 16S-ITS RFLP cluster were classified into the corresponding 16S sequencing cluster whether a 16S similarity cut-off value of 97 or 98% was used. We conclude that the taxonomic resolution of 16S-ITS RFLP fingerprinting is higher than that of 16S rDNA sequencing; this may provide a tool for reducing the number of laborious DNA-DNA hybridizations necessary for discovering potentially new species within Streptomyces.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Streptomycetaceae
/
Polimorfismo de Longitud del Fragmento de Restricción
/
Dermatoglifia del ADN
Idioma:
En
Revista:
Res Microbiol
Asunto de la revista:
MICROBIOLOGIA
Año:
2005
Tipo del documento:
Article