Metallothionein 1 isoform gene expression induced by cadmium in human peripheral blood lymphocytes.
Biomed Environ Sci
; 19(2): 104-9, 2006 Apr.
Article
en En
| MEDLINE
| ID: mdl-16827180
ABSTRACT
OBJECTIVE:
To study the gene expression of metallothionein 1 (MT-1) isoforms in human peripheral blood lymphocytes (HPBLs).METHODS:
The expression of mRNA representing the seven active MT-1 genes was determined in HPBLs by quantitative RT-PCR before and after exposure to cadmium.RESULTS:
Basal expressions of MT-1X, and MT-1A in HPBLs were similar to expression of housekeeping gene. In contrast, the basal gene expressions of MT-1H, 1F, 1E, and 1G were a little transcripts in human HPBLs. No signal was detected for MT-1B. There was a sex difference (P < 0.05). in basal gene expression of MT-1E. The levels of gene expression of MT-1A, 1E, 1F, 1G, 1H, and 1X increased, but the level of MT-1B did not increase after exposure to cadmium.CONCLUSIONS:
Gene expressions of MT-1G, MT-1H, MT-1F, and MT-1X in HPBLs can be used as a potential biomarker of cadmium exposure.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Cadmio
/
Biomarcadores
/
Regulación de la Expresión Génica
/
Isoformas de Proteínas
/
Metalotioneína
Límite:
Adult
/
Female
/
Humans
/
Male
Idioma:
En
Revista:
Biomed Environ Sci
Asunto de la revista:
SAUDE AMBIENTAL
Año:
2006
Tipo del documento:
Article
País de afiliación:
China